| A robust Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method was developed for the estimation
of Fosamprenavir calcium in pharmaceutical dosage forms. Chromatographic separation was performed on an Agilent
Zorbax SB-C18 column (4.6x250mm, 5?m), with a mobile phase comprising methanol, acetonitrile, and water in the ratio
of 40:40:20/v, at a flow rate of 1.0 ml/min, and detection at 249 nm. System suitability tests demonstrated excellent
performance, with theoretical plates at 6341 and a tailing factor of 1.78. The method showed high accuracy with a
percentage recovery of 100.6%, precision with a %RSD of 0.93%, and strong linearity with a correlation coefficient of
0.999. The limits of detection and quantification were 0.507 µg/ml and 1.538 µg/ml, respectively, indicating high
sensitivity. This method is suitable for routine analysis and quality control of Fosamprenavir calcium in oral suspension
dosage formsddd |
