| There is significant vector-borne disease spread worldwide by Culex pipiens. Insect resistance and environmental pollution
have been caused by the extensive use of synthetic insecticides. Applications of nanoparticles in insect management are a
necessary alternative control strategy. A study was carried out to assess the effects of copper oxide nanoparticles
(CuONPs) on the histological and cellular damage in larval Culex pipiens tissues. A regression log concentration-response
line was established after 24 hours of treatment to determine the lethal concentrations LC10, LC25, LC50 and LC90. Each
concentration was replicated four times with 25 third-instar larvae participating per replicate. As copper oxide
nanoparticles (CuONPs) concentrations increased for LC10, LC25, LC50, and LC90, mortality rates increased
significantly. These percentages 0.040, 0.099, 0.268, and 1.767 mg/ml were the concentrations, respectively. As a result of
this study, levels of cellular damage enzymes There are two major sources of nitric oxide and lipid peroxidase increased
significantly, whereas levels of total protein decreased significantly. Among larvae treated with increasing concentrations
of lethal CuONPs, a significant increase in albumin concentrations was observed in tissue homogenates. Furthermore,
histological studies indicated that the exposed larvae exhibited severe deterioration of their tissue architecture, which
increased with an increase in CuONP concentration. Accordingly, CuONPs cause cellular damage to Culex pipiens larvae,
deteriorating their histological structure, making them promising and effective larvicidalsddd |
