| The present work involved the development of accurate, precise, simple and suitable RP-HPLC method for estimation of
the drugs in multicomponent tablet formulations. A thorough literature survey revealed few spectrophotometric methods
were reported like, UV-Visible and colorimetric method for simultaneous estimation of these drugs in pharmaceutical
formulations.Simple, sensitive and reliable spectroscopic methods for estimation of Escitalopram and Clonazepam in
combined dosage form have been attempted. In RP-HPLC method, the analyte were resolved using KH2PO4 Buffer
(20mM): and a mixture of 50:50 of Methanol and Acetonitrile (55:45), pH 6.0 at a flow rate of 1.2ml/min, on HPLC
autosampler system containing UV- visible detector, and Photo diode array detector with LC-Solution Software and
Nucleosil C8 column (4.6 x 150 mm). The detection was carried out at 240 nm. The method gave the good resolution and
suitable retention time and an optimum suitability parameter. From the studies it can be concluded that RP-HPLC
technique can be successfully used for the estimation of Escitalopram and Clonazepam in their combined dosage tablet
formulations. The method shows good reproducibility Compared to UV-spectrophotometric methods. The RP-HPLC
method is accurate, precise, specific, reproducible and sensitive. No interference of additives, matrix etc. is encountered in
these methods. Further studies on other pharmaceutical formulations would throw more light on these studies. The methods
were found to be, rapid and economic and given optimum chromatographic parameterddd |
